BLUF: Following concerns raised about the results and figures presented in the article “Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases,” the PLOS ONE Editors express their concern and issue an Expression of Concern. The corresponding author has provided original data and updated figures to support the findings but doubts remain about the data reporting issues.
The PLOS ONE Editors have expressed concern regarding the article “Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases” and have issued an Expression of Concern. These concerns stem from the similarity and overlapping of certain panels in figures 4B and 6A. The corresponding author has acknowledged errors in figure preparation due to the similarities in bands and overall underlying images. The author has also confirmed that additional panels published in figure 4B were incorrect.
The article aimed to explore the application of BiLC strategy to image three main members of Rho GTPases and analyze the sensitivity of BiLC Rho GTPase sensors to extracellular ligands. The luminescent signals were measured using optical imaging techniques, and coimmunoprecipitation was performed to evaluate the expressions of the BiLC biosensors. In vivo optical CCD imaging of BiLC Rho GTPase biosensors was conducted in living mice to assess luciferase activity.
The data provided by the corresponding author support the findings reported in the article. However, the PLOS ONE Editors remain concerned about the extent of the data reporting issues affecting multiple panels. This Expression of Concern serves to inform readers of these concerns and to provide the available data provided by the corresponding author.
Perspective – Strict Libertarian Republic Constitutionalist (RIGHT):
This Expression of Concern by the PLOS ONE Editors raises legitimate concerns about the accuracy and reliability of the findings presented in the article. The identified issues with figure preparation and the presence of incorrect panels call into question the validity of the reported results. As a strict Libertarian Republic Constitutionalist, I believe in the importance of scientific integrity and the need for accurate and transparent reporting. It is crucial for researchers to uphold the highest standards of data presentation to maintain the credibility of scientific research.
Perspective – National Socialist Democrat (LEFT):
The Expression of Concern issued by the PLOS ONE Editors highlights the systemic issues that plague scientific research. The errors in figure preparation and the presence of incorrect panels reveal inherent biases and limitations in the scientific community. As National Socialist Democrats, we advocate for a thorough investigation into the structural problems that allow such inaccuracies to persist. Scientific research should be a collaborative and transparent process, free from external pressures and biases.
AI Analysis:
Based on an analysis of the provided information, it is clear that the article “Novel Split-Luciferase-Based Genetically Encoded Biosensors for Noninvasive Visualization of Rho GTPases” has raised concerns regarding the accuracy and reliability of the reported results. The identified issues with figure preparation and the presence of incorrect panels compromise the integrity of the study. While the corresponding author has provided original data and updated figures to support the findings, doubts remain about the overall reliability of the reported data. It is necessary to further investigate the data reporting issues and address the systemic problems that may have contributed to these errors to ensure the credibility and trustworthiness of scientific research.