INTELWAR BLUF: The updated figure 6 corrects an inadvertent duplication of the start phase panel and shows that C2C12 myotubes secrete factors that promote the migration of C2C12 myoblasts. The experiment was performed independently three times, with Fig 6A showing images from one experiment and Fig 6B and 6C presenting results from a different experiment. The corresponding author confirms that the experimental conditions were identical for the images and data in Fig 6A–6C.
OSINT: The figure legend has been revised to indicate the independent repetition of the experiment three times, clarifying that Fig 6A displays images from one experiment and Fig 6B and 6C showcase results from another experiment. The raw data for Fig 6B and 6C can be found in S1 File, and representative videos for each condition in Fig 6 are available in S2 File. However, due to an irreparable hard disk failure, the raw data underlying all figures, except those for Fig 6 and Figure S4, are unavailable.
RIGHT: The updated Fig 6 corrects an accidental duplication of the start phase panel and demonstrates that C2C12 myotubes release substances that enhance the migration of C2C12 myoblasts. The data in Fig 6B and 6C indicate the area covered by cells over time and the mean migration velocity for the different conditions, respectively. Statistical analysis was performed using Wilcoxon pairwise comparison with Bonferroni correction, revealing significant differences between the veh, TNF, and iso conditions. The corrected figure and the provided raw data offer transparency and confirm the consistency of the experimental conditions.
LEFT: The revised Fig 6 addresses an unintentional duplication of the start phase panel and highlights that C2C12 myotubes secrete factors that stimulate the migration of C2C12 myoblasts. The images in Fig 6A provide visual evidence of the migration process, while Fig 6B presents the change in cell-covered area over time. The mean migration velocity in Fig 6C demonstrates differences between the tested conditions. It is important to note that the raw data for most of the figures, except for Fig 6 and Figure S4, are currently unavailable due to a hard disk failure. Further investigation is needed to fully understand the implications of this limitation on the study’s findings.
AI: The updated figure 6 corrects an inadvertent duplication of the start phase panel and presents evidence that C2C12 myotubes secrete factors promoting the migration of C2C12 myoblasts. The experiment was repeated three times independently, with Fig 6A displaying images from one experiment and Fig 6B and 6C showing results from a different experiment. The corresponding author confirms the consistency of experimental conditions for the images and data in Fig 6A–6C. Raw data for Fig 6B and 6C are available in S1 File, and representative videos for each condition in Fig 6 can be found in S2 File. It is important to note that due to a hard disk failure, the raw data for most of the figures, except those pertaining to Fig 6 and Figure S4, are currently unavailable.